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- Hayden Christensen as Clay Beresford
- Jessica Alba as Sam Lockwood
- Terrence Howard as Dr. Jack Harper
- Lena Olin as Lilith Beresford
- Christopher McDonald as Dr. Larry Lupin
- Sam Robards as Clayton Beresford Sr.
- Arliss Howard as Dr. Jonathan Neyer
- Fisher Stevens as Dr. Puttnam
- Georgina Chapman as Penny Carver
- David Harbour as Dracula
- Steven Hinkle as Young Clay