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A DC animated film, set in the Batman: Arkham Asylum video game's universe. It takes place after the events in Batman: Arkham Origins video game. The animated movie revolves around the Suicide Squad, who are forced to steal the information from Riddler, and at the same time, assassinate him. To make their job difficult, they must break into Arkham Asylum.
- Kevin Conroy as Batman / Bruce Wayne
- Neal McDonough as Deadshot / Floyd Lawton
- Hynden Walch as Harley Quinn / Dr. Harleen Quinzel
- Matthew Gray Gubler as Riddler / Edward Nygma
- Troy Baker as Joker
- C.C.H. Pounder as Amanda Waller
- Greg Ellis as Captain Boomerang / George Harkness
- Giancarlo Esposito as Black Spider / Eric Needham
- John DiMaggio as King Shark
- Jennifer Hale as Killer Frost / Louise Lincoln
- Nolan North as KGBeast / Anatoly Knyazev, Oswald Cobblepot / Penguin
- Eric Bauza as Security Guy
- Chris Cox as Commissioner James Gordon
- Martin Jarvis as Alfred Pennyworth
- Peter Jessop as Watch Commander
- Christian Lanz as Scarecrow / Jonathan Crane
- Andrea Romano as Woman
- Travis Willingham as Morgue Guy
- Mick Wingert as Joker Security Guy