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- Jeffrey Combs as Dr. Herbert West
- Jason Barry as Dr. Howard Phillips
- Simon Andreu as Warden Brando
- Santiago Segura as Speedball
- Elsa Pataky as Laura Olney
- Lolo Herrero as Sergeant Moncho
- Enrique Arce as Cabrera
- Barbara Elorrieta as Emily Phillips
- Raquel Gribler as Nurse Vanessa