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- Jonathan Bennett as Bo Duke
- Randy Wayne as Luke Duke
- April Scott as Daisy Duke
- Adam Shulman as Enos Strate
- Willie Nelson as Uncle Jesse
- Chris McDonald as Boss Hogg
- Sherilyn Fenn as Lulu Coltrane Hogg
- Harland Williams as Sheriff Rosco P. Coltrane
- Joel Moore as Cooter Davenport
- Jennifer Hill as Brooke Handy
- Trishelle Cannatella as Ally Handy
- Kym Stys as Mechanical Bullrider
- Todd Grinnell as Hughie Hogg
- Sy Richardson as Shiner
- Vanessa Motta as Cocktail Waitress
- Challen Cates as Psychic
- Carrie Minter as The Dream Girl