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- Kate Beckinsale as Selene
- Michael Ealy as Detective Sebastian
- India Eisley as Eve
- Theo James as David
- Stephen Rea as Dr. Jacob Lane
- Kris Holden-Ried as Quint Lane
- Charles Dance as Thomas
- Sandrine Holt as Lida
- Catlin Adams as Olivia
- Adam Greydon Reid as Alan
- Scott Speedman as Michael Corvin
- Wes Bentley as Edward Vronski