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- Kate Beckinsale as Selene
- Scott Speedman as Michael Corvin
- Tony Curran as Marcus Corvinus
- Derek Jacobi as Alexander Corvinus
- Steven Mackintosh as Andreas Tanis
- Bill Nighy as Viktor
- Zita Gorog as Amelia
- Shane Brolly as Kraven
- Brian Steele as William Corvinus
- Andrew Kavadas as Selene's Father
- John Mann as Samuel
- Sophia Myles as Erika